The present work describes fast, accurate, precise, reproducible and rugged spectroscopic method for simultaneous estimation of Ondansetron Hydrochloride and Rabeprazole Sodium in Capsule formulation using simultaneous equation. The Spectroscopic method involves measurement of absorbance at 246 nm and 284 nm, wavelengths of maximum absorbance for Ondansetron and Rabeprazole, respectively. Linearity of the method was found over the range of 2-16 μg/ml for Ondansetron and over 2-20 μg/ml for Rabeprazole. The values of Limit of Detection were found to be 0.55 μg/ml and 0.67 μg/ml for Ondansetron and 0.77 μg/ml and 0.49 μg/ml for Omeprazole at 246 nm and 284 nm, respectively. Similarly, the values of Limit of Quantification were found to be 1.65 μg/ml and 2.03 μg/ml for Ondansetron and 2.33 μg/ml and 1.49 μg/ml for Omeprazole at 246 nm and 284 nm, respectively. The values of relative standard deviation for intermediate precision, intra day precision and inters day precision were found to be less than 2. Proposed method was found to be applicable for simultaneous analysis of these drugs in combined capsule formulations.
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